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KMID : 0363420080220010113
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Journal of Korean Oriental Pediatrics
2008 Volume.22 No. 1 p.113 ~ p.121
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Induction of Mitochondria-mediated Apoptosis by Solanum Nigrum in Leukemia Cells
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Jang Gyu-Tae
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Abstract
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Objectives :In human myeloid leukemia cells, there are no specific features of apoptosis compared with apoptosis in other cell types. Solanum nigrum L.(SNL) is a deciduous tree, which is widely distributed in Korea with reported anti-tumor, anti-inflammatory and non-specific immune-enhancing properties. Although the plant has been clinically used for treating a variety of diseases, its bioactive ingredients are unknown and its mode of action potential has never been investigated. Thus anti-tumor property of methanol extract was investigated.
Methods :In this study, anti-tumor property of methanol extract was investigated by determining its in vitro growth-inhibitory effects on human myeloid leukemia cells. XTT proliferation assay, DNA fragmentation, immunoblot analysis, densitometric analysis were used.
Results :1. The methanol fraction of the extracts of SNL induced mitochondria-mediated apoptosis in human myeloid leukemia cells. 2. The methanol fraction exhibited relatively higher cytotoxic activity in a dose-dependent manner than chloroform, and hexane fraction. 3. Typical ladder profile of Oligonucleosomal fragments were appeared. 4. The secreted cytosolic cytochrome C level was increased by treatment of methanol fraction.
Conclusions : Methanol fraction of SNL is capable of inducing apoptosis in human myeloid leukemia cells.
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KEYWORD
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Solanum nigrum L., Mitochondria-mediated apoptosis, human myeloid leukemia cells
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